Mouse Anti-CD29 (Fibronectin receptor subunit beta, FNRB, GPIIA, Integrin beta-1, MDF2, MSK12, VLA-4 subunit beta, VLAB, VLA-BETA)
Human CD29 is the beta subunit of an integrin family of molecules expressed on diverse cell types which function as the major receptors for extracellular matrix and as cell-cell adhesion molecules. CD29 can form heterodimer pairs with at least nine different alpha subunits.
Applications
Suitable for use in Western Blot and Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 5x10e5 cultured human HPB-MLT cells were washed and incubated 45 min on ice with 80ul of anti-CD29 antibody at a concentration of 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.3 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human ocular melanoma cell line, V+B2
Form
Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 50mM sodium chloride. No preservative added.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CD29 integrin subunit.
