Mouse Anti-Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla)
CD29 (integrin Beta-1/gpIIa) is a cell adhesion molecule appearing on platelets, as the common Beta subunit of the very late activation antigen (VLA), and as a component of various protein complexes binding to extracellular matrix proteins. The CD29 is expressed at the cell surface exclusively as part of a heterodimer, in association with one of at least nine different integrin alpha subunits (alpha1, alpha2, alpha3, alpha4, alpha5, alpha6, alpha7, alpha8, and alphav). With the exception of red blood cells and possible weak expression on granulocytes, CD29 is expressed on nearly all cell and tissue types.
Applications
Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
Flow Cytometry: 1:25-1:50 ELISA: 10ug/ml Western Blot: Reacts with human beta 1 integrin subunit under non-reducing conditions only Optimal dilutions to be determined by the researcher.
Positive Controls
Tonsil and human skin tissue
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified human beta 1 integrin preparation from HT1080 fibrosarcoma cell extract
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity
Recognizes active human beta 1 integrin. Reacts with a ligand-induced binding site, with antibody binding increasing in the presence of a fibronectin ligand. Enhances alpha 5 – beta 1 fibronectin interactions. Does not crossreact with mouse or rat.
