Mouse Anti-CD29 (Integrin beta 1) (HRP)
CD29 is also known as beta1 integrin or VLA-4 subunit alpha. CD29 is a ~130kD under reducing, ~115kD under non-reducing conditions single pass type I transmembrane glycoprotein. CD29 acts as the common beta subunit of the heterodimeric very late antigens 1-6, complexing with CD49a-f respectively where it forms part of the receptors for laminin, collagen and fibronectin. the VLA heterodimers mediate cell-cell and cell-matrix interactions.
Applications
Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation and Flow Cytometry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: frozen Optimal working dilutions to be determined by researcher.
Hybridoma
X63/Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Purified human b1 integrin preparation from HT1080 fibrosarcoma cell extract.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human beta1 integrin. Has been shown to bind to a ligand induced binding site antibody, binding being increased in the presence of fibronectin ligand. This antibody also enhances alpha 5-beta 1-fibronectin interactions. No crossreactivity with mouse or rat. Species Crossreactivity: mink
