Mouse Anti-CD29 (Integrin beta 1)
CD29 is also known as beta1 integrin or VLA-4 subunit alpha. CD29 is a ~130kD under reducing, ~115kD under non-reducing conditions single pass type I transmembrane glycoprotein. CD29 acts as the common beta subunit of the heterodimeric very late antigens 1-6, complexing with CD49a-f respectively where it forms part of the receptors for laminin, collagen and fibronectin. the VLA heterodimers mediate cell-cell and cell-matrix interactions.
Applications
Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation and Flow Cytometry. Other applications not tested.
Recommended Dilutions
ELISA: 10ug/ml Immunohistochemistry: frozen Flow Cytometry: 1:25-1:50 Optimal working dilutions to be determined by researcher.
Hybridoma
X63/Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified human b1 integrin preparation from HT1080 fibrosarcoma cell extract.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human beta1 integrin. Has been shown to bind to a ligand induced binding site antibody, binding being increased in the presence of fibronectin ligand. This antibody also enhances alpha 5-beta 1-fibronectin interactions. No crossreactivity with mouse or rat. Species Crossreactivity: mink
