C2381-10A-ML490
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
MaxLight™490Isotype
IgG1Clone Number
3H1196 (4B7R)Grade
Affinity PurifiedApplications
FC IHC IPCrossreactivity
HuShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Mouse Anti-CD29 (Integrin beta 1) (MaxLight 490)
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 10ul labels 10e6 cells in 100ul. Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin sections. Optimal dilutions to be determined by the researcher.
Recommended Secondary Reagents
I1904-65N: F(ab')2 rabbit anti-mouse IgG HRP conjugate
Recommended Negative Control
I1904-79D: Mouse IgG1 Negative Control
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Occular melanoma cell line V+B2.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the beta 1 subunit of human integrin (CD29), which is a transmembrane glycoprotein expressed by most leukocytes.
