Mouse Anti-CD29 (Integrin beta 1)
Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 10ul labels 10e6 cells in 100ul. Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin sections. Optimal dilutions to be determined by the researcher.
Recommended Secondary Reagents
I1904-65N: F(ab')2 rabbit anti-mouse IgG HRP conjugate
Recommended Negative Control
I1904-79D: Mouse IgG1 Negative Control
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Occular melanoma cell line V+B2.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the beta 1 subunit of human integrin (CD29), which is a transmembrane glycoprotein expressed by most leukocytes.
