C2383-23E
Clone Type
MonoclonalHost
RatSource
MouseConjugate
APCIsotype
IgG2a,kClone Number
390Grade
PurifiedApplications
FC IHC IPCrossreactivity
MoShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeRat Anti-CD31 (PECAM-1, Platelet gpIIa Molecule) (APC)
CD31, also known as platelet endothelial cell adhesion molecule-1 (PECAM-1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and paltelets.1-3 CD31 interacts homotypically in cell adhesion assays. This antibody inhibits the aggregation of L cells transfected with a variant form of CD31.1, 3
Applications
Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry, an Blocking of cell adhesion. Other applications not tetsted
Recommended Dilution
Flow Cytometry: ≤0.2ug/10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Mouse leukocyte cell line 32D.
Form
Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with Allophycocyanin (APC).
Specificity
Recognizes mouse CD31/PECAM-1, Mr 130-140kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Abelda, S.M. Personal communication 2. Buck, C.A., H.S. Baldwin, H. DeLisser, C. Mickanin, H.-M. Shen, G. Kennedy, A. Chen, J.M. Edelman, and S.M. Abelda. 1993. Comptes Rendus de l-Academie des Sciences Serie III-Sciences de la Vie-Life Sciences 316:838. 3. DeLisser, H.M., P.J. newman, and S.M. Abelda. 1993. Curr. Topics Microbiol. Immunol. 184:37.USBio References
No references available