Technical Data

C2392-05J
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
BE-1
Grade
Affinity Purified
Applications
E FC IP
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Mouse Anti-CD40 (B Cell surface antigen CD40, Bp50, CD40L receptor, CDw40, CDW40, MGC9013, p50, TNFRSF5, Tumor necrosis factor receptor superfamily member 5)

Human CD40 is a member of the tumor necrosis factor (TNF) receptor family and is present on all B cells except plasma cells. CD40 is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 plays an important role in B cell activation and the interaction with its ligand CD154 (CD40 Ligand) is essential for isotype switching (1). The CD40-CD154 interaction appears to have a role in artherosclerosis and cardiovascular disorders (3,4,5).

Applications
Suitable for use in ELISA, Flow Cytometry and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Flow Cytometry: 5x10e5 cultured Raji cells per tube were incubated 45 min on ice with 80ul of anti-CD40 antibody at 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.36 log10 fluorescent units when compared to a Mouse1.2 IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Murine L cells transfectants expressing human CD40
Form
Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride. No preservative added.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CD40. Binding of cross linked CD40 will partially activate B cells.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. C.L. Law, B. Wormann & T.W. LeBien, (1990) Leukemia 4:732-738, 2. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 90-91, 3. T.A. Foy, et al, (1996) Annu Rev Immunol 14:591-617, 4. M.R. Kuhné , et al, (1997) J Exp Med 186:337-342.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net