C2394-02J-ML490
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
MaxLight™490Isotype
IgG1Clone Number
3H1299 (PM6/248)Grade
Affinity PurifiedApplications
FCCrossreactivity
HuShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Mouse Anti-CD41 (CD41 Antigen, CD41b, gp2b, GPalphaIIb, GPIIb, Gta, HPA3, HPA-3, Integrin alpha IIb, Integrin alpha 2b, ITGA2B, ITGAB, Platelet Glycoprotein IIb of IIb/IIIa Complex, Platelet Fibrinogen Receptor alpha, Platelet Membrane Glycoprotein IIb, Platelet specific Antigen Bak) (MaxLight 490)
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
The CD41 cell surface antigen is a 140kD glycoprotein expressed by platelets and megakaryocytes. CD41 is also known as platelet glycoprotein IIb, and functions as a receptor for fibrinogen, fibronectin and vWF.
Applications
Suitable for use in Flow Cytometry. Not suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Hybridoma
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Human platelet plasma membrane.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human CD41. Not established if this antibody recognizes free human CD41 or human CD41 only when complexed with CD61. Antibody binding is reduced in the presence of EDTA suggesting that the epitope recognized is dependent upon an intact CD41/61 complex. Species Crossreactivity: baboon, mink and mustelid.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Hornby, E.J. et al. (1991) Activation of human platelets by exposure to a monoclonal, PM6/248, to glycoprotein IIb-IIIa. Br. J. Haematol. 79: 277-285. 2. Michelson, A.D. et al. (1995) A panel of platelet mAb for the study of haemostasis and thrombosis in baboons. Leukocyte Typing V. Oxford University Press p 1230-1231. 3. Maloney, S.F. ety al. (2010) P2Y12 or P2Y1 inhibitors reduce platelet deposition in a microfluidic model of thrombosis while apyrase lacks efficacy under flow conditions. Integr Biol (Camb). 2: 183-92. 4. Aasted, B. et al. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink. Vet Immunol Immunopathol. 119: 27-37. 5. Massoudy, P. et al. (2001) Evidence for inflammatory responses of the lungs during coronary artery bypass grafting with cardiopulmonary bypass. Chest. 119: 31-6. 6. Kahng, J. et al. (2008) Quantitative comparisons of antibody-binding sites of platelet glycoprotein IIb/IIIa in aplastic anemia and idiopathic thrombocytopenic purpura. Ann Clin Lab Sci. 38: 6-11. 7. Chae, H. et al. (2009) EDTA Inhibits the Binding of Clone 96.2C1, an Anti-CD41a Monoclonal Antibody, to the Platelets and Addition of Heparin and CaCl2 to the Antibody Neutralizes the EDTA-induced Inhibitory Effect Korean J Hematol 44: 42 - 6. 8. Zahler, S. et al. (1999) Acute cardiac inflammatory responses to postischemic reperfusion during cardiopulmonary bypass. Cardiovasc Res. 41: 722-30. 9. Wang, J.S. et al. (2005) Effects of exercise training and deconditioning on platelet aggregation induced by alternating shear stress in men. Arterioscler Thromb Vasc Biol. 25: 454-60. 10. Welsh, J.D. et al. (2012) Platelet-targeting sensor reveals thrombin gradients within blood clots forming in microfluidic assays and in mouse. J Thromb Haemost. Sep 15.USBio References
No references available