Technical Data

C2394-35A1-ML490
Clone Type
Monoclonal
Host
Mouse
Source
Sheep
Conjugate
MaxLight™490
Isotype
IgG1
Clone Number
10B1564 (CO.35E4)
Grade
Affinity Purified
Applications
FC FLISA IP
Crossreactivity
Bo Ca Eq Fe Gt Rb Sh
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Mouse Anti-CD41, CD61 (CD41b, GP2B, GP-IIb, GPIIb, GP3A, GP-IIIa, GPIIIa, GTA, HPA3, Integrin alpha 2b, ITGA2B, Integrin beta 3, ITGB3, Platelet Glycoprotein IIb of IIb/IIIa Complex) (MaxLight 490)

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

CD41/CD61 is also known as GpIIb/IIIa. CD41/CD61 is a calcium-complex dependent heterodimer and is expressed almost exclusively on platelets and on their precursors, the megakaryocytes. This complex is an activation-dependent receptor for RGD (Arg-Gly-Asp)-containing glycoproteins such as fibrinogen, fibronectin, von Willebrand factor, thrombospondin and vitronectin. It plays a central role in platelet aggregation and haemostasis.
Applications
Suitable for use in FLISA, Flow Cytometry and Immunoprecipitation. Other applications have not been tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Recommended Negative Control
I1904-78R: IgG1 Murine Negative Control
Hybridoma
Sp2-0/AG14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Sheep leukocytes
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes sheep CD41/CD61. Species Crossreactivity: goat, feline, rabbit, canine, equine and bovine

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Brodersen, R. et al. (1998) Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species: Vet. Immunol. Immunopathol. 64: 1-13. 2. Pintado, C. O. et al. (1995) Characterisation of a membrane receptor on ruminants and equine platelets and peripheral blood leucocytes similar to the human integrin receptor glycoprotein IIb/IIIa (CD41/CD61). Vet Immunol. Immunopathol. 44 (3-4): 359-368. 3. Lalko, C. et al. (2003) Equine platelet CD62P (P-selectin) expression: a phenotypic and morphologic study. Vet. Immunol. Immunopathol. 91: 119-134. 4. Major, T.C. et al. (2010) The attenuation of platelet and monocyte activation in a rabbit model of extracorporeal circulation by a nitric oxide releasing polymer. Biomaterials. 31: 2736-45. 5. Brainard, B.M. et al. (2012) Treatment with aspirin or clopidogrel does not affect equine platelet expression of P selectin or platelet-neutrophil aggregates. Vet Immunol Immunopathol. 149: 119-25. 6. Iwaszko-Simonik, A. et al. (2015) Expression of surface platelet receptors (CD62P and CD41/61) in horses with recurrent airway obstruction (RAO). Vet Immunol Immunopathol. 164 (1-2): 87-92.
USBio References
No references available
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