Technical Data

C2394-35B
Clone Type
Monoclonal
Host
Mouse
Source
Chicken
Isotype
IgG1
Clone Number
10B1565 (11C3)
Grade
Affinity Purified
Applications
FC IF IP
Crossreactivity
Ch
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CD41, CD61 (CD41b, GP2B, GP-IIb, GPIIb, GP3A, GP-IIIa, GPIIIa, GTA, HPA3, Integrin alpha 2b, ITGA2B, Integrin beta 3, ITGB3, Platelet Glycoprotein IIb of IIb/IIIa Complex)

CD41/61 is a heterodimeric cell surface protein that is expressed on chicken thrombocytes and cells of the thrombocyte lineage. Avian thrombocytes are nucleated equivalents of mammalian platelets that play a role in coagulation and phagocytosis.

Applications
Suitable for use in Flow Cytometry, Immunofluorescence, and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher.
Hybridoma
X63/A63-653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Peripheral blood leukocytes from white leghorn chicken.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the chicken integrin CD41/61. Reported to induce thrombocyte activation.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Lacoste-Eleaume, A.S. et al. (1994) Biochemical and functional characterization of an Avian homolog of the integrin GPIIb-IIa present on chicken thrombocytes. Exp. Cell. Res. 213: 198 - 209. 2. Corbel, C. et al. (2002) Expression of alpha V beta 3 integrin in the chick embryo aortic endothelium. Int. J. Dev. 46: 827 - 830. 3. Del Cacho, E. et al. (2008) Isolation of chicken follicular dendritic cells. J Immunol Methods. 334: 59-69. 4. Del Cacho, E. et al. (2009) Avian follicular and interdigitating dendritic cells: isolation and morphologic, phenotypic, and functional analyses. Vet Immunol Immunopathol. 129: 66-75. 5. Ody, C. et al. (1999) Glycoprotein IIb-IIIa is expressed on avian multilineage hematopoietic progenitor cells. Blood. 93: 2898-906. 6. Chaussé, A.M. et al. (2011) Expression of Toll-like receptor 4 and downstream effectors in selected cecal cell subpopulations of chicks resistant or susceptible to Salmonella carrier state. Infect Immun. 79: 3445-54.
USBio References
No references available
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