Technical Data

C2395-11G
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
PE
Isotype
IgG1
Clone Number
MM2/174
Grade
Purified
Applications
FC IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Mouse Anti-CD42b (PE) (Glycoprotein Ib, GPIb)

CD42b, also known as gpIb, is a two-chain membrane glycoprotein that forms a noncovalent complex with gpIX (CD42a) on the surface of platelets and megakaryocytes. Binding of the gpIb/gpIX heterodimer to von Willebrand factor bound to exposed subendothelial surfaces is essential for platelet adhesion at sites of injury.1-6

Applications
• Flow cytometry • Immunohistochemistry (frozen sections and paraffin sections) • Western blotting
Recommended Dilution
Flow Cytometry: 10ul/10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form
Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE.
Purity
Purified
Specificity
Recognizes human CD42b.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd, eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford.|2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD42a, b Section, Academic Press, New York, p. 235.|3. Thorsen, L.I., G. Gaudernack, N. Hack, J.M. Wilkinson, F. Brosstad, N.O. Solum, and N. Crawford. 1988. Brit. J. Haematol. 68:67.|4. Hornby, E.J., S. Brown, J.M. Wilkinson, C. Mattock, and K.S. Authi. 1991. Brit. J. Haematol. 79:277.|5. Berndt, M.C., X.P. Du, and W.J. Booth. 1987. Biochemistry 27:633.|6. Roth, G.J. 1992. Immunol. Today 13:100.
USBio References
No references available
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