Technical Data

C2397-76P-ML490
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
MaxLight™490
Isotype
IgG1
Clone Number
8J242 (MEM-59)
Grade
Affinity Purified
Applications
FC IHC IP WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
Mouse Anti-CD43 (T Cell Marker, Leukosialin, Sialophorin, or Leukocyte Sialoglycoprotein) (MaxLight 490)

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

CD43 is a 95-135kD type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. Expressed by platelets and at high levels on the surface of all leukocytes. It is negative on resting B lymphocytes and erythrocytes. C2397-76P activates T lymphocytes and also induces apoptosis in hemopoietic progenitor cells.
Applications
Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Paraffin sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Human T lymphocytes.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes neuraminidase-sensitive epitope on human CD43 (Leukosialin).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Stefanova, I., et al., (1989), “Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol”, Mol. Immunol., 26(2): 153-161|2. Deckert, M., et al., (1992), “CD59 molecule: a second ligand for CD2 in T cell adhesion”, Eur. J. Immunol., 22(11): 2943-2947|3. Stefanova, I., et al., (1988), “Characterization of a 95 kDa human leucocyte sialoglycoprotein: its identity with CD43, gpL115, leukosialin and sialophorin”, Folia Biol. (Praha), 34(4): 255-265|4. Leukocyte Typing IV., (1989), Knapp, W., et al., (Eds.), Oxford University Press|5. Leukocyte Typing V., (1995), Schlossman, S., et al., (Eds.), Oxford University Press|6. Alvarado, M., et al., (1995), “MEM-59 monoclonal antibody detects a CD43 epitope involved in lymphocyte activation”, Eur. J. Immunol., 25(4): 1051-1055|7. Leukocyte Typing VI., (1997), Kishimoto, T., et al., (Eds.), Garland Publishing Inc.|8. Cermak, L., et al., (2002), “Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules”, J. Biol. Chem., 277(10), 7955-7961
USBio References
No references available
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