Technical Data

C2398-03F
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
BU52
Grade
Affinity Purified
Applications
FC IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Mouse Anti-CD44 (CD44 antigen, CD44R, CDw44, CDW44, CSPG8, ECMR-III, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH-I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, Pgp1, PGP-1, PGP-I, Phagocytic glycoprotein 1, Phagocytic glycoprotein I)

Human CD44 molecules appear to serve as lymphocyte adhesion molecules with numerous functions in extracellular matrix binding, cell migration, hemopoisis, and lymphocyte homing.

Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: Formalin-fixed paraffin Flow Cytometry: 5x10e5 cultured Molt-4 human tumor cells were incubated 45 min on ice with 80ul of anti-CD44 (epitope 1) antibody at 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.98 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human peripheral myeloma cells
Form
Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride. No preservative added.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes epitope 1 of human CD44.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) p. 619-627, 2. D.J. Anstee, et al, (1991) Immunology 74:197-205, 3. B.F. Hynes, et al, (1993) Adv Immunol 54:271-335, 4. H.X. Liao, et al, (1995) J Immunol 155:3938-3945, 5. BL Rothman, TM Aune et al,(1991)J Immunol 147(8):2493-2499
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net