Mouse Anti-CD44 (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1) (PE)
Chicken CD44 is a type I transmembrane glycoprotein expressed on many cell types, including B cells, most T cells, monocytes and some epithelial cells.1 CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development.2,3
Applications
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications have not been tested.
Recommended Dilution
Immunohistochemistry: Frozen sections (acetone fixed). Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Form
Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE.
Specificity
Recognizes chicken CD44 (Mr 90kD). Also reacts with IgM+ turkey B cells and a subset of turkey T lymphocytes. Also reacts with IgM+ turkey B cells and a subset of turkey T lymphocytes.