Technical Data

C2398-14B
Clone Type
Monoclonal
Host
Mouse
Source
Chicken
Conjugate
PE
Isotype
IgG1,k
Clone Number
AV6
Grade
Purified
Applications
FC IP
Crossreactivity
Ch
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Mouse Anti-CD44 (HCAM, Homing Cell Adhesion Molecule, Pgp-1, Phagocytic Glycoprotein-1, Hermes Antigen, Lymphocyte Homing Receptor, ECM-III, HUTCH-1) (PE)

Chicken CD44 is a type I transmembrane glycoprotein expressed on many cell types, including B cells, most T cells, monocytes and some epithelial cells.1 CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development.2,3

Applications
Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications have not been tested.
Recommended Dilution
Immunohistochemistry: Frozen sections (acetone fixed). Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Form
Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE.
Purity
Purified
Specificity
Recognizes chicken CD44 (Mr 90kD). Also reacts with IgM+ turkey B cells and a subset of turkey T lymphocytes. Also reacts with IgM+ turkey B cells and a subset of turkey T lymphocytes.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Davison, T.F. Personal Communication.|2. Lazaar, A. L., and E. Pure. 1995. The Immunologist 3:19.|3. Pure, E., R.L. Camp, D. Peritt, P.A. Panettieri, A.L. Lazaar and S. Nayak. 1995. Defective phosphorylation and hyaluronate binding of CD44 with point mutations in the cytoplasmic domain. J. Exp. Med. 181:55-62.
USBio References
No references available
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