Technical Data

C2399-08P-ML650
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Conjugate
MaxLight™650
Isotype
IgG2b
Clone Number
YW62.3
Grade
Affinity Purified
Applications
FC IF IHC IP
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Rat Anti-CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) (MaxLight 650)

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

CD45 is encoded by 3 alleles in mice, differentially expressed by various inbred strains. The Ly5 gene was originally described with the gene product LY5.1 expressed in C57bl/6 and Ly5.2 expressed in SJL strains, this was subsequently expanded to include a third allele encoding Ly5.3. Further, in 1987 a reversal of nomenclature was instigated resulting in the allele in C57bl/6 becoming Ly5b encoding Ly5.2 and the allele in SJL mice becoming Ly5a encoding Ly5.1. Further changes were made in 1992 with Ly5.1 becoming CD45.1 (SJL) and Ly5.2 becoming CD45.2 (C57bl/6). Finally, following work demonstrating homology between the CD45 antigen and a receptor linked protein tyrosine phosphatase the CD45a gene was renamed Ptprca and CD45b renamed Ptprcb. A number of different isoforms isoforms of CD45 are expressed on murine leucocytes depending on the pattern of altenative splicing of 3 exons termed A, B and C encoding regions of ~ 50 amino acids located at the N terminal region of the extracellular portion of CD45. The restricted proteins are termed CD45R with a designation depending on the expressed codon product.
Applications
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Immunofluorescence. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Optimal working dilutions to be determined by researcher.
Hybridoma
Spleen cells from immunized DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLightt™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Mouse spleen cells.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the mouse CD45 cell surface antigen, a 180-220kD glycoprotein expressed by all leukocytes. Reactive with all isoforms of mouse CD45. Some reactivity with human tissue has been observed.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Watt, S.M., et al (1987). Cell surface markers on haemopoietic precursors. Reagents for the isolation and analysis of progenitor cell subpopulations. Mol. Cell. Probes 1: 297-326. 2. Bindon, C.I., et al (1988). 2. Bindon, C.I. et al. (1988) Importance of antigen specificity for complement-mediated lysis by monoclonal antibodies. Eur. J. Immunol. 18: 1507-1514. 3.. Chan, D.A. et al (2009) Tumor vasculature is regulated by PHD2-mediated angiogenesis and bone marrow-derived cell recruitment. Cancer Cell. 15: 527-38. 4. Lebson, L. et al. (2010) Trafficking CD11b-positive blood cells deliver therapeutic genes to the brain of amyloid-depositing transgenic mice. J Neurosci. 30: 9651-8.
USBio References
No references available
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