C2400-02B
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
FITCIsotype
IgG1Clone Number
MEM-28Grade
PurifiedApplications
FCCrossreactivity
HuGene ID
5788Shipping Temp
Blue IceStorage Temp
-20°CMouse Anti-CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) (FITC)
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Applications
Suitable for use in Flow Cytometry. Other applications have not been tested.
Recommended Dilution
Flow Cytometry: 20ul/100ul of whole blood or 10e6 cells in a suspension. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human thymocytes and T lymphocytes.
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity
Recognizes all alternative forms of human CD45. Does not react with equine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen: V. Horejsi, et al.; Folia Biol (Praha) 32, 12 (1986) |2. Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74): V. Horejsi, et al.; Folia Biol (Praha) 34, 23 (1988) |3. Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules: L. Cermak, et al.; J. Biol. Chem. 277, 7955 (2002)USBio References
No references available