Technical Data

C2400-05F
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Conjugate
PE/Cy5
Isotype
IgG2b
Clone Number
13/2.3
Grade
Purified
Applications
FC IHC IP
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rat Anti-CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) (PE/CY5)

CD45 is the common leukocyte antigen and is expressed on all cells of hematopoietic origin except erythrocytes.1-4 CD45 exists as a number of different isoforms which result from alternative RNA splicing of exons 4, 5 and 6. The CD45 molecule has been implicated as playing a key role in thymic development and in antigen-driven proliferation of T cells.4

Applications
• Flow cytometry 2, 3 • Immunoprecipitation 1, 3 • Radiometric quantitation of CD45 molecules on cell surfaces 1 • Immunohistochemistry (frozen sections)
Recommended Dilution
Flow Cytometry: ≤ 0.2ug/10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PE/CY5 combination conjugate is used as a third fluorochrome in multicolor flow cytometry applications when only a single 488nm excitation source is available. Antibodies are covalently coupled using heterobifunctional linking reagents, resulting in an optimal conjugation format for flow cytometry.
Form
Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE/CY5.
Purity
Purified
Specificity
Recognizes mouse CD45 (T200 or leukocyte common antigen/LCA, all isoforms). Recognizes a framework epitope present on all CD45 isoforms.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Johnson, P, L. Greenbaum, K. Bottomley and I. S. Trowbridge. 1989. J. Exp. Med. 169:1179.|2. Haidl, I. D., D. H. W. Ng, S. Rothenberger, P. Johnson, and W. A. Jefferies. 1996. Eur. J. Immunol. 25:3370.|3. Trowbridge, I.S. 1978. J. Exp. Med. 148:313.|4. Trowbridge, I.S and M.L. Thomas. 1994. Ann. Rev. Immunol.. 12:85.
USBio References
No references available
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