Technical Data

C2400-10M
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
HI30
Grade
Affinity Purified
Applications
FC IHC WB
Crossreactivity
Hu
Gene ID
5788
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CD45 (Receptor-type Tyrosine-protein Phosphatase C, Leukocyte Common Antigen, L-CA, T200, PTPRC)

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA), B220, and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution
Immunohistochemistry: paraffin Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes human CD45. Species Crossreactivity: chimpanzee.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Knapp, W., et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. 2. Kishihara, K., et al. 1993. Cell 74:143. 3. Esser, M., et al. 2001. J. Virol. 75:6173. (WB) 4. Yamada, T., et al. 2002. J. Biol. Chem. 277:28830. 5. Nagano, M., et al. 2007. Blood 110:151. 6. Jiang Q., et al. 2008. Blood 112:2858. 7. Nagano, M., et al. 2007. Blood 110:151. 8. Morozov, A., et al. 2010. Clin Cancer Res. 16:5630. 9. Yoshino, N., et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) 10. Thomas, M. 1989. Annu. Rev. Immunol. 7:339. 11. Trowbridge, I., et al. 1994. Annu. Rev. Immunol.12:85.
USBio References
No references available
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