Rat Anti-CD45R (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein)
Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:100-1:200; 10ul labels 10e6 cells in 100ul Immunohistochemistry: frozen and paraffin Optimal dilutions to be determined by the researcher.
Hybridoma
S194/5 XX0.BU-1 rat myeloma cells with spleen cells from Lewis rats.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Murine leukemia-induced pre-B tumor cells (RAW112).
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes mouse CD45R. Species Crossreactivity: human and feline.
