Technical Data

C2400-47J
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
MEM-55
Grade
Affinity Purified
Applications
FC IHC IP WB
Crossreactivity
Hu Mk
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CD45RB (CD45R, CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, PTPRC, Receptor-type Tyrosine-protein Phosphatase C Polypeptide, T200, T200 Glycoprotein)
EC=3.1.3.48

CD45RB is an of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RB is expressed e.g. in microglia and inflammatory cells.

Applications
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 0.5-2ug/ml Immunohistochemistry: No treatment of paraffin sections necessary. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human thymocytes and T lymphocytes.
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes a carbohydrate eptitope of human CD45RB, a sialidase sensative epitope which is expressed on most PBL. Species Crossreactivity: monkey

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Horejsí, V., et al., 1988, Folia Biol. 34, 23-43. 2. Bazil, V., et al., 1989, Immunogenetics 29, 2020-205.|3. Knapp, W., et al., 1989, Leucocyte Typin IV, Oxford, workshop nr. N002 Contributions No. N13.1, N16.5, N21.1, N21.2, P1.6 4. Schlossman, S., et al., 1995, Leucocyte Typing V, Oxford Univ. Press, Workshop nr. T-151, T-CD45.08.
USBio References
No references available
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