Technical Data

C2400-50H1
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Conjugate
FITC
Isotype
IgG2a,k
Clone Number
C363.16A
Grade
Purified
Applications
FC
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rat Anti-CD45RB (Leukocyte Common Antigen, LCA, Ly-5 T200) (FITC)

CD45RB represents an exon B-dependent form of CD45 glycoprotein. It is expressed at high density on peripheral B cells, the “suppressor/cytotoxic” subpopulation of T lymphocytes, a subset of “helper T cells, most thymocytes and at low density on macrophages and dendritic cells.1-4

Applications
• Flow cytometry 1-3
Recommended Dilution
Flow Cytometry: ≤ 1ug/10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Cloned mouse Th2 cell lines
Form
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified
Specificity
Recognizes an exon B-dependent epitope of CD45 glycoprotein.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Bottomly, K., M. Luqman, L. Greenbaum, S. Carding, J. West, T. Pasqualini, and D.B. Murphy. 1989. Eur. J. Immunol. 19:617.|2. Hathcock, K.S., G. Laslo, H.B. Dickler, S.O. Sharrow, P. Johnson, I.S. Trowbridge, and R.J. Hodes. 1992. J. Immuol. 148:19.|3. Johnson, P., L. Greenbaum, K. Bottomly, and I.S. Trowbridge. 1989. J. Exp. Med. 169:1179.|4. Birkeland, M.L., et al. 1988. J. Mol. Cell. Immunol. 4:71.
USBio References
No references available
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