Mouse Anti-CD45RC (MaxLight 490)
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Immunohistochemistry: frozen Optimal dilution determined by the researcher.
Recommended Secondary Reagents
Goat anti mouse IgG:HRP (rat adsorbed) Goat anti mouse IgG:RPE (rat adsorbed)
Recommended Negative Controls
I1904-78Y IgG1, Murine Negative Control (For rat tissue)
Hybridoma
Spleen cells from immunized BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
PHA stimulated rat lymphocytes
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes rat CD45C., the high molecular weight form of the leucocyte common antigen found on B cells, approximately 50% of bone marrow cells, all CD8+ve T cells but splits CD4+ve T cells into two populations differing in their helper activities.
