Mouse Anti-CD46 (MCP, Membrane Cofactor Protein)
CD46 functions as an inhibitor of complement activation limiting the formation and activity of C3 convertases. CD46 is expressed by all nucleated cells, often as multiple isoforms on the same cells. The molecule is also expressed by sperm and may be important in the process of fertilization.
Applications
Suitable for use in Flow Cytometry, Western Blot, Immunofluorescence and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:10-1:50. Use 10ul to label 10e6 or 100ul whole blood. Western Blot: non-reducing conditions Optimal working dilutions to be determined by researcher.
Recommended Secondary Antibody
F(ab')2 rabbit anti mouse IgG: RPE conjugate
Recommended Negative Controls
Mouse IgG2a Negative Control
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
HPB-ALL cell line.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the human CD46 cell surface antigen, a broadly expressed glycoprotein also known as membrane co-factor protein (MCP).
