MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
Applications
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence and Western Blot. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo
Recommended IHC Positive Control
Tonsil
Recommended Negative Control
I1904-78R: IgG1 Murine Negative Control
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Burkitt’s lymphoma cell line Raji
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes the human 45kD leukocyte surface glycoprotein specific for the CD48 antigen which is closely related to the activation antigen, Blast-1. It is useful in the detection of CD48 positive cells and may be used in studies to differentiate haemapoietic tumors, sarcomas and melanomas. |Species Crossreactivity: Rhesus monkey