Mouse Anti-CD48
Applications
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence and Western Blot. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 2ug/ml Immunohistochemistry (Paraffin): 5-10ug/ml Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies
I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Burkitt’s lymphoma cell line Raji
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes the human 45kD leukocyte surface glycoprotein specific for the CD48 antigen which is closely related to the activation antigen, Blast-1. It is useful in the detection of CD48 positive cells and may be used in studies to differentiate haemapoietic tumors, sarcomas and melanomas. |Species Crossreactivity: Rhesus monkey