Suitable for use in Flow Cytometry, Inhibition and Immunohistochemistry. Other aapplications not tested.

Inhibition: Inhibits SCC9 cell adhesion to human and EHS laminin, but not laminin peptide GD-2. Potentiates inhibition of SKOV-3 cell adhesion to EHS laminin by anti-integrin alpha 5 antibody GoH3. Does not inhibit either cell’s adhesion to fibronectin or collagen type IV. Immunohistochemistry: On acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue. Flow Cytometry: Stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelial cells (HUVEC). Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Human squamous cell carcinoma cells (SCC9)

Supplied as a liquid in PBS pH 7.6, 0.09% sodium azide.

Purified by Protein A affinity chromatography.

Recognizes human alpha-3 integrin (CD49c).