Technical Data

C2404-71D
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IP WB
Crossreactivity
Hu
Gene ID
3676
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-CD49d (Integrin alpha4)

Integrins are alpha/beta heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 alpha and 8 beta subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2).

Applications
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to residues surrounding Ser1027 of human integrin alpha4.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity
Purified by peptide affinity chromatography.
Specificity
Recognizes endogenous levels of human integrin alpha4 mature protein (150kD), alpha4 precursor protein (140kD), as well as 70kD cleaved C-terminal alpha 4 fragment.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2:91–100. 2. Liu, S. et al. (2000) J Cell Sci 113(20):3563–71. 3. Plow, E.F. et al. (2000) J Biol Chem 275:21785–8. 4. Bonder, C.S. et al. (2005) Immunity 23:153–63. 5. Arroyo, A.G. et al. (1999) Immunity 11:555–66. 6. Yang, J.T. et al. (1995) Development 121:549–60. 7. Nishiya, N. et al. (2005) Nat Cell Biol 7:343–52. 8. Alon, R. et al. (2005) J Cell Biol 171:1073–84. 9. Lanzarotto, F. et al. (2006) Drugs 66:1179–89. 10. Bartt, R.E. (2006) Curr Opin Neurol 19:341–9. 11. Teixidó, J. et al. (1992) J Biol Chem 267:1786–91. 12. Pujades, C. et al. (1996) Biochem J 313(3):899–908.
USBio References
No references available
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