C2405-03E
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG2b,kClone Number
5K227 (4F10)Grade
PurifiedApplications
FC IHC IPCrossreactivity
HuShipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
Mouse Anti-CD49f (Laminin Receptor, Integrin alpha6, VLA alpha6, Platelet gpIc)
CD49f is the 125 kDa integrin α6 chain which associates noncovalently with the integrin β1 subunit (CD29) and with the integrin β4 (CD104) chain to form, respectively, the very late antigen-6 (VLA-6) and α6β4 integrin heterodimers. VLA-6 is expressed on thymocytes, T lymphocytes, monocytes, and platelets. CD49f/CD29 expression is upregulated on activated T lymphocytes and memory T cells. VLA-6 serves as the laminin receptor on platelets, monocytes, and T cells. VLA-6-mediated binding to laminin provides a costimulatory signal to T cells for activation and proliferation.
Applications
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Flow Cytometry: ≤ 1ug/10e6 cells Immunohistochemistry: frozen sections Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
SW1222 human colorectal cell line
Form
Supplied as a liquid in 100mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Specificity
Recognizes human CD49f.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Hemler, M.E. 1990. Annu. Rev. Immunol. 114:365. 2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD49f Section, Academic Press, New York, p. 267. 3. Tamura, R.N., H.M. Cooper, G. Callo, and V. Quaranta 1991. Proc. Natl. Acad. Sci. USA 88:10183. 4. Chen N et al, J Biol Chem 279 (42): 44166-44176 (2004). 5. Bae JS et al., Biochem Biophy Res Comm 294: 940-948 (2002). 6. Furumatsu T et al, J Biochem (Tokyo) 131: 619-626 (2002). 7. Ahmed N et al, J Cellular Biochemistry 84: 675-686. (2002).USBio References
No references available