C2405-03P
Clone Type
MonoclonalHost
RatSource
MouseIsotype
IgG2a,kClone Number
GoH3Grade
Affinity PurifiedApplications
FC IC IPCrossreactivity
Bo Ca Eq Fe Hu Mo Po Rb ShShipping Temp
Blue IceStorage Temp
-20°CRat Anti-CD49f (Integrin alpha-6, CD49 Antigen-like Family Member F, VLA-6, ITGA6)
CD49f is a 120kD integrin family member, also known as VLA-6 α chain and α6 integrin subunit. CD49f associates with either integrin β1 (CD29) or integrin β4 (CD104) to form receptors (VLA-6 or α6β4 complex) for laminin and kalinin. CD49f is expressed on platelets, monocytes, T cells, placental trophoblasts, epithelial and endothelial cells. CD49f is involved in adhesion and can act as a co-stimulatory molecule for T cell activation and proliferation.
Applications
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Mouse mammary tumor cells
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes mouse CD49f. Species Crossreactivity: baboon, cynomolgus, rhesus, equine, bovine, sheep, porcine, canine, feline, rabbit and human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Georas, S. N., et al. 1993. Blood 82:2872. 2. Honda, T., et al. 1995. J. Clin. Endocrinol. Metab. 80:2899. 3. Sonnenberg, A., et al. 1986. J. Histochem. Cytochem. 34:1037. 4. Nakamura, K., et al. 1997 Biochem. Biophys. Res. Commun. 235:524. 5. Sonnenberg, A., et al. 1987 J. Biol. Chem. 262:10376. 6. Deregibus, M.C.., et al. 2007. Blood. doi:10.1182/blood-2007-03-078709. 7. Horwitz, K.B., et al. 2008. Proc Natl Acad Sci U S A. 105:5774. 8. Nardella, C., et al. 2009. Sci Signal. 2:55. 9. Xu, T., et al. 2010. Mol Cancer Ther. 9:438. 10. Stepp, M.A., et al. 2007. J Cell Sci. 120:2851. 11. Jo, M., et al. 2010. Cancer Res. 70:8948. 12. Yoshino, N., et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) 13. Sonnenberg A., et al. 1990. J. Cell. Biol. 110:2145. 14. Sonnenberg, A., et al. 1990. J. Cell. Sci. 96:207. 15. Aumailly, M.R., et al. 1990. Exp. Cell Res. 188:55. 16. Niessen, C. M., et al. 1994. Exp. Cell Res. 211:360.USBio References
No references available