Technical Data

C2405-05S
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2b
Clone Number
186-2G9
Grade
Affinity Purified
Applications
FC IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CD50 (CDw50, CDW50, ICAM-3, ICAM-R, Intercellular adhesion molecule 3)

Human CD50 is an adhesion molecule that interacts with LFA-1 (CD18/CD11a) and provides a costimulatory signal for T cells (1, 2, 3) which is thought to be essential for initiation of immune responses. Recognizes the D1 domain of the ICAM-3 molecule of 130kD (4), and blocks binding of CD11a (LFA-1) to CD50 (ICAM-3).

Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: Paraffin Flow Cytometry: 5x10e5 cultured human Jurkat cells per tube were washed and pre-incubated 5 min on ice with 20ul of 250ug/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of anti-CD50 antibody at 10ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.84 log10 fluorescent units when compared to a Mouse IgG2b negative control at a similar concentration. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Stimulated human leukocytes
Form
Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CD50.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford (1995) p. 1546-1547, 1578-1579, 2. C.L. Holness, et al, (1995) J Biol Chem 270:877-884, 3. Y. van Kooyk, et al, (1996) J Exp Med 183:1247-1252, 4. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 403-409.
USBio References
No references available
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