C2408-11A-ML550
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
MaxLight™550Isotype
IgG1Clone Number
3H1544 (MEM-53)Grade
Affinity PurifiedApplications
FC IHC IP WBCrossreactivity
HuShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Mouse Anti-CD53 (MaxLight 550)
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
CD53 cell surface antigen is also known as Tetraspanin-25. CD53 is a 219 amino acid multi pass transmembrane glycoprotein of 32-42 kDa. CD53 is expressed by all leucocytes, but is absent from erythrocytes and platelets.
Applications
Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: non-reducing conditions Immunohistochemistry: Frozen sections Optimal working dilutions to be determined by researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the human CD53 cell surface antigen.
