Technical Data

C2410-50A
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
143-30
Grade
Purified
Applications
FC IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Mouse Anti-CD55 (DAF, Decay Accelerating Factor)

CD55, also known as decay accelerating factor (DAF), is a 64-73kD GPI-anchored single chain glycoprotein that is expressed on all cells in contact with serum, including all hematopoietic cells and the vascular endothelium. A soluble form of CD55 is present in plasma and body fluids. CD55 is a member of the regulator of complement activity (RCA) family of proteins and is thought to play a role in the protection of cells from damage by autologous complement by preventing the amplification steps of the complement cascade. CD55 expression increases upon T cell activation and antibodies to CD55 are mitogenic in the presence of phorbol esters.1-5

Applications
Suitable for use in Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: ≤ 1ug/10e6 cells Immunohistochemistry: frozen Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PHA activated peripheral blood mononuclear cells
Form
Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Purity
Purified
Specificity
Recognizes human CD55.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Knapp, W., B. Dorken, W.R. GIlks, E.P. Rieber, R.E. Schmidt, H. Stein, A.E.G.K. Von dem Borne, eds. 1989. Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, Oxford.|2. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD55 Section, Academic Press, New York, p. 281.|3. Lublin, D.M., and J.P. Atkinson. 1989. Annu. Rev. Immunol. 7:35.|4. Holmes, C.H., K.L. Simpson, S.D. Wainwright, C.G. Tate, J.M. Houlihan, I.H. Sawyer, I.P. Rogers, F.A. Spring, D.J. Anstee, and M.J. Tanner. 1990. J. Immunol. 144:3099.|5. Liszewski, M.K., T.C. Farries, D.M. Lublin, I.A. Rooney, and J.P. Atkinson. 1996. Adv. Immunol. 61:201.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net