Technical Data

C2412-05L
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
Biotin
Isotype
IgG1
Clone Number
TS2/9.1.4.3
Grade
Affinity Purified
Applications
FC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CD58 (Ag3, FLJ23181, FLJ43722, LFA3, LFA-3, Lymphocyte function-associated antigen 3, Surface glycoprotein LFA-3) (Biotin)

Human CD58 is a cell surface glycoprotein that functions as an adhesion molocule and mediates costimulatory activity through its ligand CD2, which is expressed on T and natural killer cells (1,2). CD58 has wide cellular distribution on both hemopoietic and non-hemopoietic cells with strong expression on macrophages and memory T cells.

Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 5x10e5 cultured human Nalm-6 cells were washed and incubated 45 min on ice with 80ul of anti-CD58/Biotin at 10ug/ml. Cells were washed twice and incubated with Streptavidin/R-PE after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.1 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre-incubated 10 min with 20ul of 0.5mg/ml anti-CD58 antibody. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human cytolytic T cells
Form
Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 500mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with biotin.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes the CD58 glycoprotein of about 60-70kD. Inhibits HLA-DR mediated T cell cytotoxicity.|

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 414-415, 2. A.R. Arulanandam, et al, (1994) J Exp Med 180:1861-1871.
USBio References
No references available
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