Mouse Anti-CD58 (Ag3, FLJ23181, FLJ43722, LFA3, LFA-3, Lymphocyte function-associated antigen 3, Surface glycoprotein LFA-3) (Biotin)
Human CD58 is a cell surface glycoprotein that functions as an adhesion molocule and mediates costimulatory activity through its ligand CD2, which is expressed on T and natural killer cells (1,2). CD58 has wide cellular distribution on both hemopoietic and non-hemopoietic cells with strong expression on macrophages and memory T cells.
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 5x10e5 cultured human Nalm-6 cells were washed and incubated 45 min on ice with 80ul of anti-CD58/Biotin at 10ug/ml. Cells were washed twice and incubated with Streptavidin/R-PE after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.1 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre-incubated 10 min with 20ul of 0.5mg/ml anti-CD58 antibody. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human cytolytic T cells
Form
Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 500mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with biotin.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes the CD58 glycoprotein of about 60-70kD. Inhibits HLA-DR mediated T cell cytotoxicity.|