Mouse Anti-CD59 (MACIF, MIRL, Protectin) (Biotin)
Applications
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions
Flow Cytometry: 1-2ug/ml Immunohistochemistry: paraffin Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Thymocytes and T lymphocytes
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with Biotin.
Specificity
Recognizes a well defined epitope (W40, R-53) on CD59 (Protectin), an 18-20kD glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on the surface of all hematopoietic cells; it is widely present on cells in all tissues.
