Mouse Anti-CD62L (CD62 Antigen-like Family Member L, gp90 MEL, hLHRc, L Selectin, L-Selectin, LSEL, Leukocyte Adhesion Molecule 1, LAM1, LAM-1, LEU8, Leukocyte Endothelial Cell Adhesion Molecule 1, LECAM1, LECAM-1, Leukocyte Surface Antigen Leu-8, Lymph Node Homing Receptor, LNHR, Lymphocyte Adhesion Molecule 1, LYAM1, PLNHR, Selectin L, SELL, TQ1) (FITC)
Leukocyte Surface Antigen Leu8
CD62L, also known as L-selectin and LECAM-1, is a 74-94kD type I glycoprotein expressed on neutrophils, monocytes, T and B cell subsets and NK cells. A soluble form of CD62L is present at high levels in blood. Like other selectins, CD62L mediates the initial attachment and rolling of leukocytes on endothelial surfaces which is the prerequisite for leukocyte extravasation from the blood into tissues. More specifically, CD62L is important for the homing of naive lymphocytes via high endothelial venules to peripheral lymph nodes and Peyer’s patches. It also contributes to the recruitment of leukocytes to areas of inflammation.
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: neat, 10ul labels 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher.
Positive Control
Human tonsil
Hybridoma
NS1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PHA stimulated lymphoblasts.
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human CD62L. Expressed by B and T lymphocytes, monocytes and granulocytes. Species Crossreactivity: Cynomolgus monkey, Rhesus monkey, canine and bovine.
