Technical Data

C2419-06D
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
FITC
Isotype
IgG1
Clone Number
10.1
Grade
Affinity Purified
Applications
FC
Crossreactivity
Hu Mk
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CD64 (FcgRI) (FITC)

Human CD64 is a high affinity receptor for monomeric human IgG1 and IgG3 which is expressed on macrophages, monocytes, and gamma interferon induced neutrophils. CD64 plays an important role in clearance of immune complexes and in antibody dependent cytoxicity. CD64, also known as FcgRI, is a 71kD type I transmembrane protein. It is expressed exclusively on monocytes and macrophages and binds monomeric human IgG with high avidity. CD64 is thought to play a role in the antibody-dependent cytotoxicity (ADCC) reactions, phagocytosis and mediates the release of IL-1, IL-6 and TNF-alpha. INF-gamma increases the expression of CD64 and induces its expression on neutrophils which do not usually express CD64. Glucocorticoids have also been reported to increase this receptor.

Recommended Negative Controls: Mouse IgG1 Negative Control: FITC
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: Neat-1:10, use 10ul to label 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher.
Hybridoma
Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human monocytes.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human CD64. Species Crossreactivity: Clone is reported to crossreact with Cynomolgus monkey (2), baboon and Rhesus monkey

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Dougherty, G. et al. (1987). Eur. J. Immunol. 17: 1453-1459.2. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fa
USBio References
No references available
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