C2420-90-ML490
Clone Type
MonoclonalHost
RatSource
HumanConjugate
MaxLight™490Isotype
IgG2aClone Number
3H2379 (YTH71.3)Grade
Affinity PurifiedApplications
FC IHCCrossreactivity
Hu MkShipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Rat Anti-CD66acd (MaxLight 490)
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
CD66acd is expressed by human neutrophils and cells in some cases of colon carcinoma. Reacts with transfectants containing BGPa, NCA and CGM1a which are CEA subfamily members.
Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: Frozen and paraffin sections Optimal dilutions to be determined by the researcher.
Hybridoma
Y3/Ag1.2.3 myeloma cells with spleen cells from DA rats.
Recommended Negative Control
I1904-83M1: IgG2a, Rat Negative Control
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Human lymphocytes.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human CD66acd. Species Crossreactivity: Rhesus monkey. Does not react with mouse.
