Hamster Anti-CD69 (Early Activation Antigen CD69, 5830438K24Rik, AI452015, AIM, VEA)
CD69 is a 60kD type II membrane protein composed of a 27/33kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3 and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection. This antibody has been reported to augment T cell activation.
Applications
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Flow Cytometry: ≤1ug/10e6 cells in 100ul volume Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Mouse dendritic epidermal T cell line Y245
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by affinity chromatography.
Specificity
Recognizes mouse CD69.
