C2426-02G
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
PEIsotype
IgG1Clone Number
MEM-75Grade
PurifiedApplications
FCCrossreactivity
HuGene ID
7037Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeMouse Anti-CD71 (CD71 Antigen, p90, T9, Transferrin Receptor, TFR, TR, Transferrin Receptor Protein 1, TFR 1, TFR1, TFR-1, TFRC, TRFR) (PE)
CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 20ul labels human blood cells in 100ul whole blood. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Pre-B cell line NALM-6.
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity
Recognizes human CD71. Does not block binding of transferrin to the receptor.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Beck Z, Balogh A, Kis A, Izsépi E, Cervenak L, László G, Bíró A, Liliom K, Mocsár G, Vámosi G, Füst G, Matko J: New cholesterol-specific antibodies remodel HIV-1 target cells' surface and inhibit their in vitro virus production. J Lipid Res. 2010 Feb;51(2):286-96.|2. Sutherland DR, Richards SJ, Ortiz F, Nayyar R, Benko M, Marinov I, Illingworth A: CD71 improves delineation of PNH type III, PNH type II, and normal immature RBCS in patients with paroxysmal nocturnal hemoglobinuria. Cytometry B Clin Cytom. 2019 Nov 8. doi: 10.1002/cyto.b.21853.USBio References
No references available