Technical Data

C2426-02H
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
MEM-75
Grade
Affinity Purified
Applications
FC IC IP
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CD71 (CD71 Antigen, p90, T9, Transferrin Receptor, TFR, TR, Transferrin Receptor Protein 1, TFR 1, TFR1, TFR-1, TFRC, TRFR)

CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron.

Applications
Suitable for use in Flow Cytometry, Immunocytochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Flow Cytometry: 4ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Pre-B cell line NALM-6
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human CD71. Does not block binding of transferrin to the receptor.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Leucocyte Typing IV, ed. W. Knapp et al., Oxford Univ. Press, Oxford 1989: Workshop nr A45. And 5th workshop (Mab no. T-165; requested CD71 standard) Serveral contributions to 4th and 5th workshops. 2. Horejsí, V., et al., 1986, Fol. Biol. 34, 12. 3. Leucocyte Typing V., Schlossman S. et al., 1995, Oxford University Press.
USBio References
No references available
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