Technical Data

C2431-05C
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
Biotin
Isotype
IgG1,k
Clone Number
5K219 (CB3-1)
Grade
Purified
Applications
FC IP WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CD79b (BCR-Ig beta) (Biotin)

CD79 is a disulfide-linked heterodimer composed of an α chain (CD79a) and a β chain (CD79b) that associates non-covalently with membrane immunoglobulin to form the B cell receptor (BCR) complex. Its expression is restricted to B lymphocytes first appearing on the surface at the pro-B cell stage and remaining through all stages of B cell differentiation prior to plasma cells. Crosslinking of the BCR leads to B cell activation.

Applications
Suitable for use in Flow Cytometry, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended Dilution
Flow Cytometry: 10ul/10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Ramos B cells
Form
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Biotin.
Purity
Purified
Specificity
Recognizes the beta chain of human CD79.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd, eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford.|2. Kishimoto, T., A.E.G. von dern Borne, S.M. Goyert, D.Y. Mason, M. Miyasaka, L. Moretta, K. Okumura, S. Shaw, T.A. Springer, K. Sugamura, and H. Zola, eds. 1998. Leukocyte Typing VI: White Cell Differentiation Antigens, Academic Press, New York.|3. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD79 Section, Academic Press, New York, p. 332.|4. Nakamura, T., H. Kubagawa, and M.D. Cooper, 1992. Proc. Natl. Acad. Sci. USA 89:8522.
USBio References
No references available
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