Technical Data

C2431-05D
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
PE
Isotype
IgG1
Clone Number
CB3-1
Grade
Purified
Applications
FC IHC IP
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Mouse Anti-CD79b (BCR-Ig beta) (PE)

CD79 is a 23/21 disulfide-linked heterodimer composed of an alpha chain (CD79a/mb-1) and a beta chain (CD79b/B29) that associates non-covalently with membrane immunoglobulin to form the B cell receptor (BCR) complex. Its expression is restricted to B lymphocytes, first appearing on the surface at the pro-B cell stage and remaining through all stages of B cell differentiation prior to plasma cells. Crosslinking of the BCR leads to B cell activation.

Applications
• Flow cytometry • Immunohistochemistry (frozen sections) • Immunoprecipitation
Recommended Dilution
Flow Cytometry: 10ul/10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Form
Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE.
Purity
Purified
Specificity
Recognizes human CD79beta.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Schlossman, S., L. Bloumsell, W. Gilks, J.M. Harlan, C. Kishimoto, J. Ritz, S. Shaw, R. Silverstein, T. Springer, T.F. Tedder, and R.F. Todd, eds. 1995. Leukocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, Oxford.|2. Kishimoto, T., A.E.G. von dern Borne, S.M. Goyert, D.Y. Mason, M. Miyasaka, L. Moretta, K. Okumura, S. Shaw, T.A. Springer, K. Sugamura, and H. Zola, eds. 1998. Leukocyte Typing VI: White Cell Differentiation Antigens, Academic Press, New York.|3. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD79 Section, Academic Press, New York, p. 332.|4. Nakamura, T., H. Kubagawa, and M.D. Cooper, 1992. Proc. Natl. Acad. Sci. USA 89:8522.
USBio References
No references available
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