Mouse Anti-CD79A (IGA, MB1, B Cell Antigen Receptor Complex-associated Protein alpha Chain, Ig-alpha, MB-1 Membrane Glycoprotein, Membrane-bound Immunoglobulin-associated Protein, Surface IgM-associated Protein) (PE)
CD79A, also known as Iga and MB-1, is a 44kD transmembrane glycoprotein in the immunoglobulin superfamily. It contains a single Ig-like domain in its extracellular domain (ECD) and one cytoplasmic immunoreceptor tyrosine-based activation motif (ITAM). Alternate splicing generates a short isoform with a 39aa deletion in the ECD. Heterodimers of CD79A and CD79B/IgB associate with a membrane bound immunoglobulin on the B cell surface to form the B cell antigen receptor complex (BCR). CD79A and CD79B are required for BCR-mediated signaling and consequently for the development and activation of B lineage cells. Within the ECD, human CD79A shares 57% aa sequence identity with mouse and rat CD79A.
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 10ul to label 1x10e6 cells using human peripheral blood lymphocytes. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Recombinant protein corresponding to Leu33-Arg143 from human CD79A expressed in NS0 cells. Species Sequence Homology: mouse and rat, 57%
Form
Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes human CD79A. No crossreactivity with recombinant mouse CD79A is observed.
