Mouse Anti-CD80 (T-lymphocyte Activation Antigen CD80, Activation B7-1 Antigen, BB1, CTLA-4 Counter-receptor B7.1, B7, CD28LG, CD28LG1, LAB7) (Biotin)
Human CD80 (B7-1) is a costimulatory ligand for CD28 and CTLA-4. CD80 is expressed on activated B cells. C2432-01F blocks binding of soluble CD152 Ig fusion protein to CD80.
Applications
Suitable for use in Flow Cytometry and ELISA. Other applications not tested.
Recommended Dilution
Flow Cytometry: 80ul at 5ug/ml labels 5x10e5 cells. Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol
1.Five x 10e5 cultured Raji cells were washed and incubated 45 minutes on ice with 80ul C2432-01F at 5ug/ml. 2. Cells were then washed twice, incubated with secondary reagent. 3. Cells were then washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.81 log10 fluorescent units when compared to a Mouse IgM/FITC negative control at a similar concentration. 4. Binding was blocked when pre incubated 10 minutes with 20ul of 0.5mg/ml C2432-01E.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human CD80 (B7-1) Ig Fusion Protein.
Form
Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CD80.
