Rat Anti-CD80 (CD80 Antigen, CD80 Molecule, Activation B7-1 Antigen, B-lymphocyte Activation Antigen B7, B7, BB1, CD28 Antigen Ligand 1 B7-1 Antigen, CD28LG, CD28LG1, Costimulatory Factor CD80, CTLA-4 Counter-receptor B7.1, LAB7, T lymphocyte Activation Antigen CD80)
CD80 (B7-1) is a 60kD cell surface glycoprotein which is a member of the CD28/B7 family. In mice, CD80 is expressed on monocytes, peritoneal macrophages and dendritic cells, and expression may be significantly increased upon B lymphocytes by LPS and by IL-4. CD80 has been identified as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4), two structurally similar molecules expressed on T cells. CD28 and CTLA4 are two receptors that have essential but opposing functions in T-cell stimulation. The Interaction of CD80 with CD28 stimulates and sustains T cell responses, whereas the interaction of CD80 with CTLA4 is reported to inhibit T-cell responses.
Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Hybridoma
P3U1 myeloma cells with spleen cells from SD rats.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
BCL1 cells expressing CD80.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes mouse CD80.
