C2439-60M
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG1Clone Number
6A515 (W17/1)Grade
Affinity PurifiedApplications
FC IHCCrossreactivity
HuShipping Temp
Blue IceStorage Temp
-20°CMouse Anti-CD88 (C5a Receptor, C5aR)
Human anaphylatoxin CD88 is a 74-residue glycopolypeptide which is generated by proteolytic cleavage of the complement factor C5 in the course of complement activation. A variety of biological effects evoked by CD88 could be demonstrated, rendering this molecule an important mediator of inflammation, with granulocytes and macrophages as the main target cells. All cellular responses to CD88 are specifically mediated by interactions with the membrane bound C5a receptor, a seven transmembrane GTP-binding-protein-coupled receptor that belongs to the rhodopsin supergene family.
Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Not suitable for use in Immunoprecipitation or Western Blot. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:10 Immunohistochemistry (Frozen, paraffin): 1:10 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human CD88 at ~45kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Opperman, M et al; Probing the human receptor for C5a anaphylatoxin with side-directed antibodies. J Immunol 1993, 151: 3785. 2. Opperman, M et al; Antibodies from the myeloid panel that react with the C5a receptor and antagonize C5a biological activity. Schlossman, S.F. (ed.) Leucocyte typing V.O.U.P.: 955-956. 3. Werfel, T et al; Binding of anti-C5a receptor (C5aR) antibodies to cells of clinically normal human skin. Schlossman, S.F. (ed.) Leucocyte typing V.O.U.P.: 957-959. 4. Opperman, M et al; Plasma clearance of human C5a anaphylatoxin by binding to leucocytes C5a receptors. Immunol 1994, 82: 516.USBio References
No references available