Suitable for use in Flow Cytometry. Other applications not tested.

Flow Cytometry: ≤0.5ug/10e6 cells in 100ul Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

MV4-11 acute monocyte leukemia cells.

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purified by affinity chromatography

Recognizes human CD92. Specific for the C-­terminal variant of human CDw92 (isoform 3), also known as CTL1, a 70kDa multi­pass membrane protein, expressed by monocytes, neutrophils, certain myeloid and T cell lines, and weakly by endothelial cells, fibroblasts and epithelial cells. Reported to augment the LPS ­induced production of IL­10 by monocyte­ derived dendritic cells (Mo­ DCs); the reduced expression of CDw92 by Mo­DCs treated with ionomycin or calcium ionophore, can be reinduced in the presence of IL­10 (Wille et al.).