Technical Data

C2445-25AX
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgM
Clone Number
7H103
Grade
Affinity Purified
Applications
FC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Mouse Anti-CD100 (CD100 Antigen, A8, BB18, C9orf164, Coll-4, GR3, M-sema-G, SEMAJ, Semaphorin-4D, SEMA4D)
FLJ33485, FLJ34282, FLJ39737, FLJ46484, MGC169138, MGC169141, Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D

Human CD100 is a transmembrane glycoprotein that belongs to the semaphorin gene family and is found on most hematopoietic cells except RBC, platelets and immature bone marrow cells. CD100 plays a key role in lymphocyte activation (2, 3).

Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
PHA stimulated human PBL
Form
Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 50mM sodium chloride. No preservative added.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CD100 at ~150kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 534-539, 2. K.T. Hall, et al, (1996) Proc Natl Acad Sci USA 93:11780-11785, 3. A. Elhabazi, et al, (1997) J Biol Chem 272: 23515-23520, 4. S. Mizrahi, O Mandelboim, et al.(2007) PLoS ONE 2(9):e818.
USBio References
No references available
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