Technical Data

C2445-39H
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
FITC
Isotype
IgG1
Clone Number
BB27
Grade
Affinity Purified
Applications
FC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CD101 (CD101 Molecule, Cell Surface Glycoprotein V7, EWI101, EWI-101, Glu-Trp-Ile EWI Motif-containing Protein 101, Immunoglobulin Superfamily Member 2, IgSF2, V7) (FITC)

CD101 is a 140kD homodimeric cell surface glycoprotein expressed primarily by monocytes, granulocytes, dendritic cells and activated T lymphocytes.

Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:5-1:10, 10ul labels 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human thymic clone B12
Form
Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant
Specificity
Recognizes human CD101. Reported to inhibit allogeneic T-cell responses (ref. 1)

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Bagot, M. et al. (1997). CD101 is expressed by skin dendritic cells. Tissue Antigens. 50: 439-448.|2. Jovanovic, D.V. et al. (2011) CD101 expression and function in normal and rheumatoid arthritis-affected human T cells and monocytes/macrophages. J Rheumatol. 38: 419-28.|3. Ohradanova-Repic, A. et al. (2016) Differentiation of human monocytes and derived subsets of macrophages and dendritic cells by the HLDA10 monoclonal antibody panel. Clin Transl Immunology. 5 (1): e55.|4. Okuno, M. et al. (2017) Nucleotide substitutions in CD101, the human homolog of a diabetes susceptibility gene in non-obese diabetic mouse, in patients with type 1 diabetes. J Diabetes Investig. 8 (3): 286-94.|Grassi, F. et al. (1998) Monocyte-derived dendritic cells have a phenotype comparable to that of dermal dendritic cells and display ultrastructural granules distinct from Birbeck granules. J Leukoc Biol. 64 (4): 484-93.
USBio References
No references available
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